Troponin-H expression.

1 dimensional SDS-PAGE showing expression and purification of recombinant Drosophila troponin-H isoform 34.

Key to lanes:

1 & 2 Total cell proteins from an un-induced culture.
3 & 4 Total cell proteins 45 and 90 min post-induction.
5 & 6 Detergent insoluble and soluble protein fractions.
   7 Soluble fraction flow through of after application to a Tn-H affinity column.
8 - 11 Elution of purified Tn-H from the affinity column.